SAF-LAB EQUIPMENTS, FUNCTION AND USES

1. NAME OF EQUIPMENT : GAS CHROMATOGRAPHY - MASS SPECTROMETER (GCMS)

Photo: SAF LAB Utra Modern Gas Chromatography –Mass Spectrometer (GCMS)

FUNCTIONS/USES

• Analysis of volatile compounds in food molecules
• Determination of trace levels of contamination of pesticides
• Quantitative and qualitative analysis of Esters, Fatty Acids, Alcohols Aldehydes, Terpenes
• Pesticide Residue Analysis
• Protein Identification
• Carbon Dating
• Analysis of biological samples -Urine, Saliva, Plasma,
• Analysis of environmental samples such as water, Food products such as beverages,
• Authentification of food samples to prevent fraud
• Forensic drug identification analysis
• Detectable Drugs in Urine – Cocaine -(100ng/Ml), Colchicine - 10mcg/Ml); Codeine-10ng/Ml),; Nicotine-100ng/Ml),
• Screening Blood and Urine for Acute Overdose of counter medications
• Food contamination detection
• Drug Testing and Discovery
• Full scan for mass fragments range from m/z 50- m/z 600 (M/Z=mass divide by charge number)

GCMS  ACCESSORIES

• GC Mainframe
• GC Column Oven
• GCMS System Split/Splitless Injectors
• Mass Spectrometer-MS
• FID detector
• GCMS System Auto-sampler
• GCMS - Data Management and Acquisition System – Computer; Laser printer
• GCMS System - Software System

2.NAME OF EQUIPMENT : ATOMIC ABSORPTION SPECTROPHOTOMETER-(AAS)

Photo: SAF LAB Atomic Absorption Spectrophotometer-AAS

FUNCTIONS/USES

• Detect heavy metals concentration (nano-grams) (parts per billion) in solid or liquid samples-very sensitive
• Food and beverages samples – measures heavy metals concentrations
• Heavy metals concentration in the environmental, marine and geological samples
• Environmental testing of concentration of various elements in rivers, drinking water, seawater
• Heavy metals concentration in biological samples such as an human and animal tissues
• Heavy metals concentration soils, fertilizer and plant tissue samples
• Heavy metals concentration herbicides, pesticides, rodenticides and fumigants
• Mining operations - identify and measures heavy metal concentration
• Heavy metals /poisons detection in water
• Clinical research and pharmaceutical drugs - composition/quantity/quality of raw ingredients used manufacturing drugs
• Works by quantitative analysis of elements in liquid or solids samples through electromagnetic radiation. Analysis by is by molecular photo absorption.

AAS Accessories

• AAS System Optics
• AAS System Data Processing system
• AAS System Burner Unit
• AAS System Gas Control unit
• AAS System Graphite Furnace
• AAS System Auto sampler
• AAS System Accessories- Hydride Vapor Generator; air compressor; gases with regulators

3.NAME OF EQUIPMENT: BENCH TOP FREEZE DRYER

Photo: SAF LAB Bench Top Freeze Dryer

FUNCTION/USES

• Preservation of high quality perishable foods
• To extend shelf life of the food and quality
• Preservation of flavours
• Used to increase shelf life of vaccines and injectables
• For valued samples - research microbial cultures, soil samples, food materials and pharmaceuticals
• Freeze drying/ lyophilization is a drying technology- removes moisture from raw frozen products through vacuum system by process called sublimating.

SPECIFICATIONS

• Condenser lowest temperature -50⁰C (80^OC optional) CFC-free Refrigeration
• Type :EBT-12N Multi-manifolds type : main machine, 2L/S Vacuum Pump, multi-manifolds drying apparatus (4 sample tray, 8 Lyophilized bottle, 8 rubber valve)
• Tray Size : 200mm Four Layered

4.NAME OF EQUIPMENT: ROTARY EVAPORATOR

Photo: SAF LAB Rotary Evaporator

FUNCTIONS/USES

• Removes solvents with low boiling points such as ethyl acetate and n-hexane
• Removes solvents a sample by evaporation
• Used for extracting, distillation, concentration solvent, evaporation and purification

SPECIFICATIONS:

Rotary Evaporator

• Diagonal Rotary evaporator
• Specifications:
• Dimensions(WxDxH):730x410x630mm
• Power: 220V 50/60Hz 2.5A
• Flask Supplied: 1000ml
• Included: Water bath (PTFE Coating)

 Vacuum Pump

• Diaphragm Vacuum pump
• Suitable for Air, Gases and vapors
• Technical features:
• 100% oil-free
• Pure transfer,evacuation and compression
• Chemically resistant

Cooling Water Circulator (Bath)

• Cooling water circulator
• Specifications:
• Capacity: 4L
• Bath Dimension(DxH): Ø190x200
• Circulator Pump: Magnet 20W Max. flow 15L/min
• Temp Range: Down to -20˚C

5.NAME OF EQUIPMENT : MICROBIOLOGY FUME HOOD

Photo: SAF LAB Microbiology Fume Hood

FUNCTIONS/USES

• For Microbiology Research
• Protect users from inhaling toxic fumes
• Protect experiment or sample from contamination
• Protect users from explosions or spills
• Works by pulling away air from the user into the enclosure with a blower
• Actively protects operator from inhaling toxic vapors and dramatically reduces the risk of fire and explosion

SPECIFICATIONS:

• External Size (W*D*H): 1200*840*2150mm
• Internal Size (W*D*H): 1080*730*745mm
• Work Surface Height: 750mm
• Max Opening: 520mm
• Air Velocity: 0.3 - 0.8m/s
• Noise: ≤68dB
• Exterior material: Cold-rolled steel with anti-bacteria powder coating.

6.NAME OF EQUIPMENT: BIOSAFETY CABINET CLASS –II

Photo: SAF LAB Biosafety Cabinet Class –II

FUNCTIONS/USES

• Suitable for working with microbiological research in the absence of volatile or toxic chemicals and radionuclide
• Protect lab workers /users from hazardous materials using directional air flow
• The sterile air bathes and protects work from air borne contaminants
• Protects environment against harmful biological agents
• Prevent biological materials ,(cell cultures microbiological stocks) inside the BIOSAFETY CABINET CLASS –I1 from being contaminated

SPECIFICATIONS:

• Airflow system: 70% air recirculation, 30% air exhaust.
• Internal Size (W*D*H): 1150*600*660 mm
• External Size (W*D*H): 1300*750*2250mm
• Working height: 8 inches
• Inflow Velocity: 0.53±0.025 m/s
• Down flow Velocity: 0.33±0.025 m/s
• HEPA Filter:Two, 99.999% efficient at 0.3 um. Filter life indicator
• Noise: EN12469 ≤ 58 dB / NSF49 ≤ 61 dB
• UV LAMP: 30W* 1
• Display: LCD display: exhaust filter and downflow filter pressure, filter and UV lamp working time, inflow and downflow velocity, filter life, humidity and temperature, system working hours etc.
• Control System: Microprocessor
• Airflow System: 70% air recirculation, 30% air exhaust
• Visual and audio alarm: Filter replacement, window over height, abnormal air flow velocity.

7.NAME OF EQUIPMENT: UV-VIS - SPECTROPHOTOMETER SYSTEM

Photo: SAF LAB UV-VIS-Spectrophotometer System with Accessories.

FUNCTIONS/USES

• Measuring absorbance of a sample as a function of wavelength of electromagnetic radiation
• Quantitative analysis of molecular and ionic species in a solution
• Determining concentrations of compounds-
• Determining structure of a compounds
• Finding functional groups of compounds
• Determining molecular weight of a compounds
• Determining the composition of materials
• Used in forensic sciences - determining concentrations of compounds
• Used in molecular biology research - quantify molecules for nucleic acids, proteins and fats

SPECTROPHOTOMETER ACCESSORIES

• Wavelength range and spectral bandwidth = 190nm to 1100nm
• Photometric system Double beam
• Printer compatible with the UV
• Complete PC having Windows 10, 15’ LED screen , 500GB Hardisk, 4GB RAM, Mouse etc.
• PC-UV operation software

8.NAME OF EQUIPMENT: NAME OF EQUIPMENT : BINOCULAR OLYMPUS MICROSCOPE

Photo: SAF LAB Binocular Olympus Microscope

FUNCTIONS/USES

• High Magnification – (40-1000x)
• Observe enlarge and generate an image from a sample put on a slide
• Provide enlarged images of distant objects
• Ease of viewing
• Cuts down eye strain
• For parasitology research

SPECIFICATIONS:

• Binocular Microscope
• Nosepiece: Quadruple revolving nosepiece, rotation on ball bearings
• Eyepiece: Wide field eyepieces WF10X/18 with field number 18.
• Objectives: Achromatic objectives with mechanical tube length 160 mm
• Stage: Double layer with mechanical sliding stage, size 125x115mm, X-Y movement range 70x30, specimen holder for one slide.
• Supplied with: Instruction manual and dust coverExterior material: Cold-rolled steel with anti-bacteria powder coating.

9.NAME OF EQUIPMENT: FLUORESCENT MICROSCOPE

Photo: SAF LAB Fluorescent Microscope

FUNCTIONS/USES

• Uses fluorescence light to generate images form a specimen
• Uses fluorescence as a detection method instead of regular illumination and magnification
• Provides detailed features of cells and tissues
• Used in diagnostic and microbial ecology for enumerating bacteria in natural environments
• To observe living matter at the point where fluorescence dyes are present
• Used in biology/molecular biology/chemistry/physiology/and imaging
• Cell labeling-identify and label cell structures; Live cell imaging; Protein characterization

SPECIFICATIONS

• Head: Trinocular (fixed 50/50), 30° inclined, 360° rotating.
• Interpupillary distance: Adjustable between 48 and 75 mm.
• Dioptric adjustment: On the left eyepiece tube.
• Eyepieces: WF10x/20 mm, high eye-point and secured by screw.
• Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
• Objectives: IOS N-PLAN 4x/0.10;IOS N-PLAN 10x/0.25;IOS N-PLAN 20x/0.40
• Objectives: IOS N-PLAN 40x/0.65; IOS N-PLAN 100x/1.25 (Oil/Water)
• Specimen stage: Double layer rackless mechanical stage, 233×147 mm, 78×54 mm X-Y range.
• Focusing: Coaxial coarse (adjustable tension) Condenser: Abbe N.A. 1.25, with objective-coded iris diaphragm, focusable and centerable.
• Transmitted illumination (Fixed Koehler type): X-LED3 with white 3.6 W LED (6,300K) with brightness control.

10.NAME OF EQUIPMENT: INVERTED MICROSCOPE

Photo: SAF LAB Inverted Microscope

FUNCTIONS/USES

• Inverted microscope used for observing cells and organisms in petri dishes, microplates and roller bottles
• Inverted microscope used for observing living cells or organism at the bottom of a large container such as a tissue culture flask instead of a glass slide as is the case with a conventional microscope
• No need to embed the samples;No need to cut out parts of a bigger sample
• Saves times and money
• Inverted microscopes used cell culture and tissue culture as cells grow underneath the stage of the microscope –specimens can be viewed from below at the bottom

SPECIFICATIONS

• Head: Trinocular (2-position 100/0, 50/50), 45° inclined.
• Interpupillary distance: Adjustable between 50 and 75 mm.
• Dioptric adjustment: On the left eyepiece tube.
• Eyepieces: WF10x/22 mm, high eye-point and with rubber cups.
• Nosepiece: Quintuple revolving nosepiece, rotation on ball bearings.
• Objectives: IOS LWD W-PLAN PH 10x/0.25; IOS LWD W-PLAN PH 20x/0.40; IOS LWD W-PLAN PH 40x/0.65
• Specimen stage: Fixed stage, 250×160 mm, with glass and metal stage inserts.
• Focusing: Coaxial coarse (adjustable tension) and fine focusing mechanism with limit stop to prevent the contact between objective and specimen.
• Condenser: LWD pre-centered condenser, N.A. 0.30, W.D. 72 mm. With 4x/10x, 20x/40x phase contrast slider and bright field. Removable to extend the working distance to 150 mm.
• Transmitted illumination: X-LED8 with white 8 W LED (6,300K) with brightness control.

11.NAME OF EQUIPMENT: DISSECTING MICROSCOPE

Photo: SAF LAB Dissecting Microscope

FUNCTIONS/USES

• To view three dimensional (3Dimages) objects and larger specimens
• A stereoscope for viewing surface features of a specimen

SPECIFICATIONS

• Digital stereozoom microscope 7x…45x, halogen incident & transmitted illumination, with 1.3Mpixels.
• "Illumination: Dual illuminator adjustable for transmitted and reflected light, intensity control with two knobs on the right side of the base. The illumination angle in reflected light is adjustable.
• Two halogen bulbs 12V/15W provided"
• Magnification: 7x/45x
• Head: Binocular 360° rotating and 45° inclinated
• Objectives: 0,7 .... 4,5x Zoom
• Resolution: 1280 x 1024 pixels (1.3 M pixels)
• "Included with the camera:
• Optika Vision® software package, 1.5 m USB cable.

12.NAME OF EQUIPMENT: ELECTRONIC ANALYTICAL WEIGHING BALANCE

Photo: SAF LAB Electronic Analytical Weighing Balance

FUNCTIONS/USES

• Analytical Weighing Balance

SPECIFICATIONS

• Capacity: 220g
• Readability: 0001g (0.1mg)
• Material of Body: Metal
• Supplied with: Power adapter and user manual

13.NAME OF EQUIPMENT: MAGNETIC STIRRER

Photo: SAF LAB Magnetic Hot Plate Stirrer

FUNCTIONS/USES

• Used in chemistry and biology to stir and mix small volumes of solutions

 SPECIFICATIONS

• Hot plate stirrer, ceramic coated plate.
• Range: Max 380°C
• Plate Size (mm): 180x180
• Stirring capacity: 20 lts

14.NAME OF EQUIPMENT: VORTEX MIXER

Photo: SAF LAB Multifunction Vortex Mixer Set

FUNCTIONS/USES

• To mix small vials of liquids

SPECIFICATIONS

• Multifunction Vortex Mixer Set
• Speed Range: 0 ~ 3,300 rpm
• Power: AC 230V, 50/60Hz
• Dimensions: 260x200x220
• Supplied with: Pop-off Cup Head and Φ76mm (3 inch)

15.NAME OF EQUIPMENT: WATER DISTILLATION SYSTEM

Photo: SAF LAB Water Distillation System

FUNCTIONS/USES

• Laboratory distilled water

SPECIFICATIONS

• Water Still, 4l/hr single distiller 240V
• Output Capacity: 4 litres/hr- Single distilled
• pH: 5.6 -6.0
• Conductivity: 3-4
• Power Rating: 3kW

16.NAME OF EQUIPMENT: AUTOCLAVE

Photo: SAF LAB Autoclave

FUNCTIONS/USES

• Sterilizing of surgical equipment, lab instruments, pharmaceutical items

SPECIFICATIONS

• Top-loading Sterilizer with digital fuzzy control, Electronic door lock, steam condensing mechanism, lever-type sliding door, solids / liquids modes, CE-MDD Certified
• Capacity: 100L
• Temperature Range: Ambient 5℃ to 132℃
• Power Supply: 230v, 60Hz
• Heating Power: 2kW X 2
• Supplied with: 2 SUS Wire Baskets

 17.NAME OF EQUIPMENT : HOT AIR OVEN

FUNCTIONS/USES

• Drying and sterilizing

 SPECIFICATIONS

• Natural Convection oven.
• Temperature range Ambient +5°C up to +300 °C
• 53 litres volume

18.NAME OF EQUIPMENT: VACUUM FILTRATION APPARATUS

Photo: SAF LAB Vacuum Filtration Apparatus

FUNCTIONS/USES

• Removing macromolecules, colloids, emulsified oil, endotoxins, viruses and bacteria

SPECIFICATIONS

• Oil free Vacuum Pump
• 3 place Stainless steel Manifold
• 3 x Stainless Steel Funnel 300ml
• Torch Burner
• 4000 ml PP Waste Bottle with drain
• MCE Membrane, 47 mm, 0.45 μm, 200/PK
• Silicone Tube (1 x 2 Pcs)

19.NAME OF EQUIPMENT : FLASK SHAKER FOR MIXING

Photo: SAF LAB Flask shaker

FUNCTIONS/USES

• Flask shaker for mixing solvents

SPECIFICATIONS

• Material Stainless Steel
• Voltage220 / 230 V
• Frequency50/60 Hz
• Power Source Electric
• Phase Single Phase

20.NAME OF EQUIPMENT: STAINLESS STEEL TROLLEY

Photo: SAF LAB Stainless Steel Trolley

Specification:

• Dimension :- 500mm x 500mm x 950mm
• Specifications:- Welded Stainless Steel Construction – no rivets
• Wheels:- 100mm Grey Rubber swivel

Accessories

• 125mm grey rubber wheels
• Infection control fully welded
• Total locking castors
• Buffer disks
• Guard rails on all sides
• 1 or 2 Drawers
• Key locks to drawers

 21.NAME OF EQUIPMENT: LIQUID NITROGEN CONTAINER

Photo: SAF LAB Liquid Nitrogen Container

Functions/Uses

• For cryogenic storage of biological material at cryogenic temperature right from semen to stem cells both in vapor and liquid

Specification:

• TA55 Liquid Nitrogen Container
• Capacity (litres) : 51.5
• BIS certified for IS:11552 standard

22.VISITING SCIENTISTS - PH.D STUDENTS WORKING DESKS

Photo: SAF LAB Ample Visiting Scientist Desks with Internet Connectivity

23.NAME OF EQUIPMENT: COOL BOXES AND REFRIGERATORS

Photo: SAF LAB cool boxes and refrigerators

24.NAME OF EQUIPMENT: COMPACT BENCH CENTRIFUGE

Photo: SAF LAB Compact Bench Centrifuge

FUNCTIONS/USES

• Concentrate or separate substances suspended in liquid media by density
• Applications: DNA/RNA research; tissue culture, protein work, cell harvesting

SPECIFICATIONS

• Speed: 6800 rpm
• RCF: 4445 xg
• Volume: 8 x 15 ml RB / 4 x 15 ml Falcon
• Speed range: 200 - 6800 rpm
• Running time: 59 min 50 s / 10 s increments 99 h 59 min / 1 min increments
• Dimensions (WxHxD): 28 x 24 x 35 cm

25.NAME OF EQUIPMENT: TISSUE HOMOGENIZER AND EXTRACTOR

Photo: SAF LAB Tissue Homogenizer and Extractor

FUNCTIONS/USES

• Tissue Homogenizer And Extractor

26.NAME OF EQUIPMENT: NATURAL CONVECTION INCUBATOR

Photo: SAF LAB Natural Convection Incubator

FUNCTIONS/USES

• Provides temperature controlled environment to support growth of microbiological cultures

Specifications:

• Temperature range Ambient +5°C up to +70 °C
• 53 Litres Volume
• Double Doors Standard.

27.NAME OF EQUIPMENT: COOLED INCUBATOR

Photo: SAF LAB Cooled Incubator

FUNCTIONS/USES

• Provides temperature below the ambient temperature as low as+10⁰C
• Used in biology and microbiology
• Microbiological cultures, hematology, enzyme digestion studies,
• Coliform determination, tissue culturing

SPECIFICATIONS:

• Temperature range Ambient +0°C up to +70 °C
• 53 Litres Volume
• Double Doors Standard.

28.NAME OF EQUIPMENT: SHAKING WATER BATH

Photo: SAF LAB Shaking Water Bath

FUNCTIONS/USES

• For thawing, heating, mixing, and shaking samples
• Continuous shaking of microbiological samples enables liquid grown cell cultures to continuously mix with air Flask shaker for mixing solvents
• Designed to deliver precise temperature controlled ad a smooth reciprocal horizontal shaking motion

29.NAME OF EQUIPMENT: REFRIGERATED HIGH SPEED CENTRIFUGE

Photo: SAF LAB Refrigerated High Speed Centrifuge

FUNCTIONS/USES

• For separating heat labile/temperature sensitive samples or mixtures
• Able to generate speed of 60000g
• Useful to collect microorganisms, cellular debris, large cellular organelles, and proteins

 

Implementation Milestones of the SAF LAB since Inception

Objective 1 and 2: To procure, install and commission equipment at the SAF- LAB

Duration:  June 2019 - June, 2022.

Implementation Milestone